'harmless' PCR test

Jan-Henner Wurmbach jhwurmbach at gmx.de
Mon Dec 7 15:47:05 EST 1998


On Mon, 7 Dec 1998 15:10:05 +0000, tph at mrc-lmb.cam.ac.uk (Tony Hodge)
wrote:

[snip]

>On a related topic - we have an annual 'Take your Daughters to Work' day
>where daughters of staff get to come in to the lab and do some
>interesting but 'non-hazardous' science. (This year they did some EM and
>image reconstruction) Something like the above proposal could be fun
>(with proper ethical precautions) BUT - how to run a gel without using
>hazardous chemicals to visualise the DNA...
>
>any suggestions?
>
>tony

You could try DAPI-Staining of the agarose-gel. There was a thread
about that here not too long ago.
Sensitivity seems to be OK and no one had heard about hazards. But the
high price of the substance could become problematic.

greetings
  Jan-Henner Wurmbach (jhwurmbach at uke.uni-hamburg.de)




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