Amplification of Plasmid Library
Mr. M.J. Lush
mlush at hgmp.mrc.ac.uk
Mon Dec 7 09:47:28 EST 1998
I'm making a cDNA expression library in a plasmid vector.
I plan to divide the library into 125 pools of about 5000 clones each
during the amplification stage (this is because I'm screening via a
Methods in Enzymology (Vol152 1987 pp407-415) suggests that
the library should be grown in soft agar on LB agar plates to
minimize differential growth between colonys.
Whats the simplest method of seperating the bugs from the agar
(MiE suggests something fiddly involving converting the agar into a paste
by squerting through a 21 gague hypodermic needle and spinning through
a Sephadex column).
Are there any better methods lurking out there?
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