bioinfo at bioinfo at
Mon Dec 7 03:40:42 EST 1998

In article <199812070136.JAA13419 at>,
  zxn at PENTIUM.WHIOV.AC.CN (zxn) wrote:
> Dear sir:
>  I want to get a special biological software which can be used to design
> primer sets
>  in Multiplex-PCR.
> In Multiplex-PCR ,we apply several different templates just in one tube ,
> if the templates are very similar to each other ,then a primer for template
> A  may also
> can attach to template B,how can I avoid this problem and improve the
> specifity of the
> primers in these reactions ?Does there have software suit for this
> I will appreciate your information very much!
> Best regards!
> Tao
> ------------------------------------------------------
> Wuhan Institute of Virology
> Academia Sinica
> Wuhan
> P.R.China
> 430071
> ---------------------------------------------------------
With respect to multiplexing, try using Primer Premier (site: It has given me satisfactory results. For more
infomation regarding troubleshooting PCR using softwares send e-mail to
bioinfo at
Ravi Gupta

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