reconbinant resistant to any digestion

Yihua Han hanyihua at Uni-Hohenheim.DE
Tue Dec 8 13:26:27 EST 1998


Hello,everybody,
I have made a plasmid using HindIII and XhoI for insert to B-Gal-Basic and
I checked by PCR and it gave the positive result,but after that I try to
use HindIII and XhoI to cut the insert out,I couldn't get it and moreover
I found the plasmid cann't be digestied by any enzyme.By the way I use the
kit from QIAGEN to purify the plasmid.Does anyone can give any advice?many
thanks
Han





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