protocolle for 10 to 100 mg of plasmid
JUAN MORALES GRILLO
Juan.Morales at CIGB.EDU.CU
Tue Dec 8 21:36:33 EST 1998
Please, I am looking for a IN HOUSE protocolle of plasmid purification in
order to get 10 to 100 mg of plasmid for inmunization whith DNA,
using chromatografic step like ion exchange, gel filtration, or any
think that one can scale up a massive plasmid purification.
Also if someone know about references bibliografic where i could find
ideas related, i acept coment about, or advances and so on.
I need to scale up the my maxiprep procedure in order to began a
protocolle of DNA inmunization in monkeys.
Thanks for read, juan
Center for Genetic Engineering and Biotechnology.
Hepatitis C Department.
PO Box 6162, ZP 10600
Cubanacan, Ciudad Habana, Cuba.
Fax: (53-7)218070 Phone: 53-7-218008/218446/218164
E-mail: Juan.Morales at cigb.edu.cu
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