protocolle for 10 to 100 mg of plasmid

JUAN MORALES GRILLO Juan.Morales at CIGB.EDU.CU
Tue Dec 8 21:36:33 EST 1998


HALLO NETTERS:
Please, I am looking for a IN HOUSE protocolle of plasmid purification in 
order to get 10 to 100 mg of plasmid for inmunization whith DNA, 
using chromatografic step like ion exchange, gel filtration, or any 
think that one can scale up a massive plasmid purification.
Also if someone know about references bibliografic where i could find 
ideas related, i acept coment about, or advances and so on.
I need to scale up the my maxiprep procedure in order to began a 
protocolle of DNA inmunization in monkeys.
Thanks for read, juan  
Center for Genetic Engineering and Biotechnology.
Hepatitis C Department.
Vaccines Division.
PO Box 6162, ZP 10600
Cubanacan, Ciudad Habana, Cuba.
Fax: (53-7)218070 Phone: 53-7-218008/218446/218164
E-mail: Juan.Morales at cigb.edu.cu





More information about the Methods mailing list