Sample jumping out of the well?

Gina Berardesco gbe392 at merle.acns.nwu.edu
Wed Dec 9 09:35:06 EST 1998


This also will happen if you accidently use 10X TBE instead of 1 or 0.5X as
the running buffer. Not that I've ever done anything that silly.

-Gina


Guy Hoelzer (hoelzer at med.unr.edu) wrote:
> A student of mine just had a discouraging lab experience that I have seen
> before, but I do not have a good explanation.  When he loaded his samples
> into a gel, they jumped out of the wells and dispersed into the running
> buffer.  Why does this happen and how can it be prevented?  I suspect that
> there was ethanol remaining in the samples, because they were precipitated
> immediately prior to loading.  Of course, my student assures me that he
> rinsed with 70% EtOH and dried them thoroughly before disolving in TE and
> adding loading dye.  Another difference this time around was the use of
> TAE instead of TBE for electrophoresis.  Is TAE more dense than TBE? 
> Please send advice to me by email (hoelzer at med.unr.edu).

> Thanks,

> -- 
> Guy A. Hoelzer                 phone:  702-784-4860
> Department of Biology          fax:  702-784-1302
> University of Nevada Reno      email:  hoelzer at med.unr.edu
> Reno, NV  89557



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