Reagent suppressing long PCR product?

Chris Boyd chrisb at hgu.mrc.ac.uk
Thu Dec 10 07:42:14 EST 1998


Yinhua Zhang (yz66 at columbia.edu) wrote:
: Hi, there, 
: 	Does anyone known there is a chemical that can reduce the
: efficiency of long PCR product (say 3kb) but relatively has no effect on
: short PCR product (say 1kb)?   The PCR for these two product uses the same
: pair of primers.  Thanks for advices.

Have you tried reducing the extension time?

Best wishes,
-- 
Chris Boyd                      | from (but not \  MRC Human Genetics Unit
Christopher.Boyd at hgu.mrc.ac.uk  | on behalf of) /      Crewe Rd, Edinburgh
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd          EH4 2XU, SCOTLAND



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