Reagent suppressing long PCR product?
chrisb at hgu.mrc.ac.uk
Thu Dec 10 07:42:14 EST 1998
Yinhua Zhang (yz66 at columbia.edu) wrote:
: Hi, there,
: Does anyone known there is a chemical that can reduce the
: efficiency of long PCR product (say 3kb) but relatively has no effect on
: short PCR product (say 1kb)? The PCR for these two product uses the same
: pair of primers. Thanks for advices.
Have you tried reducing the extension time?
Chris Boyd | from (but not \ MRC Human Genetics Unit
Christopher.Boyd at hgu.mrc.ac.uk | on behalf of) / Crewe Rd, Edinburgh
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd EH4 2XU, SCOTLAND
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