notice of cloning problem

[Frederik Boernke]

John Richard Seavitt wrote:
> 
> On Wed, 9 Dec 1998, Bernard P. Murray, PhD wrote:
> 
> > But they do (at least for some enzymes).  Check out the NEB catalogue
> > (eg. pages 258 and 259 in the '98/'99 catalogue) for data for many
> > of the common enzymes.  There are quite a few that don't cut well
> > with a 4 bp extension but can be pursuaded to perform a little letter
> > with an extended incubation.
> 
> It's also been my experience that if the specific enzyme or overhang
> length in question isn't in the catalog, they're often able to provide
> additional information over the phone if you ask ... seems there's always
> new info waiting for the next edition.
> 
> John


There was this article in Biotechniques 24:582-584 (April 1998)where
they tested how many additional nucleotides are necessary for cutting
and non of the tested enzymes needed more than 3.

Ricky
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******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de