notice of cloning problem

Frederik Boernke boernke at
Thu Dec 10 15:38:40 EST 1998

John Richard Seavitt wrote:
> On Wed, 9 Dec 1998, Bernard P. Murray, PhD wrote:
> > But they do (at least for some enzymes).  Check out the NEB catalogue
> > (eg. pages 258 and 259 in the '98/'99 catalogue) for data for many
> > of the common enzymes.  There are quite a few that don't cut well
> > with a 4 bp extension but can be pursuaded to perform a little letter
> > with an extended incubation.
> It's also been my experience that if the specific enzyme or overhang
> length in question isn't in the catalog, they're often able to provide
> additional information over the phone if you ask ... seems there's always
> new info waiting for the next edition.
> John

There was this article in Biotechniques 24:582-584 (April 1998)where
they tested how many additional nucleotides are necessary for cutting
and non of the tested enzymes needed more than 3.



Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515

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