berg at xs4all.nl
Thu Dec 10 05:49:23 EST 1998
molecular indexing is a method that can be used to "index" cDNA form
different disease states of variuos tissues.
VERY short protocol:
first ds cDNA is digested by an class IIs restriction enzyme, then linkers
are ligated which divided the digested cDNA into 64 subpopulations. next a
pcr is performed which only amplifies the 5' end (poly T tail) of the
cDNA by use of an anchored oligo d(T) primer its possible to further
subdivide the cDNA into 3X64 = 192 subpopulations.
for a detailed protocol see also the molecular indexing site :
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