How to use Betain in PCR

bradley turner turner at sprcore.bidmc.harvard.edu
Thu Dec 3 11:22:13 EST 1998


Hi,

The following references discuss the use of Betaine (and other
additives) in PCR.  Basically, using a 5M stock solution of Betaine,
adjust to a final concentration of 0.5-1.5M in your PCR reaction.

Nucleic Acids Research 25(19)3957-3958, 1997
Betaine improves the PCR amplification of GC-rich DNA sequences
W Henke, K Herdel, K Jung, D Schnorr, S Loening

BioTechniques 21(6)1102-1108, 1996 December
Strategy for controlling preferential amplification and
avoiding false negatives on PCR typing
T Weissensteiner, J Lanchbury

Genome Research 6:633-638, 1996
Uniform amplification of a mixture of deoxyribonucleic acids
with a varying GC content
N Baskaran, R Kandpal, A Bhargava, M Glynn, A Bale, S Weissman


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                    Bradley Turner
                Beth Israel Deaconess
                    Medical Center

Harvard Medical School          617-667-1215 phone
Division of Gastroenterology    617-667-2767 fax
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Boston, MA 02215                turner at sprcore.bidmc.harvard.edu
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