Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Thu Dec 10 12:28:34 EST 1998
Has anyone any info. on a pUC12 derivative, pUC12N, modified at the lacZ
ATG to introduce an Nco I site and possibly other modifications. I have
a plasmid from years ago with an insert that I now need and I can't PCR
it with universal (-40) and reverse (-48) primers. Looking at pUC12
those primer sites should still exist but I get no PCR of the 2kb insert
(which is definitely in there according to RE digests). I have a
reference for a J. Biotechnol., Vol 2, pp157-175 (1985) but I can't find
any trace of that journal in three university libraries tried to date.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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