pUC12N

Roland Hübner rhubner at gins.uia.ac.be
Thu Dec 10 16:41:00 EST 1998


In article <vw3jdBACTAc2Ewn3 at genesys.demon.co.uk>, "Dr. Duncan Clark"
<Duncan at genesys.demon.co.uk> wrote:

>  Hi Folks,
>  
>  Has anyone any info. on a pUC12 derivative, pUC12N, modified at the lacZ
>  ATG to introduce an Nco I site and possibly other modifications. I have
>  a plasmid from years ago with an insert that I now need and I can't PCR
>  it with universal (-40) and reverse (-48) primers. Looking at pUC12
>  those primer sites should still exist but I get no PCR of the 2kb insert
>  (which is definitely in there according to RE digests). I have a
>  reference for a J. Biotechnol., Vol 2, pp157-175 (1985) but I can't find
>  any trace of that journal in three university libraries tried to date. 
>  
>  Duncan

 Dear Duncan,

 maybe one of those could help you further? Seems as of ccatgg is the only
official change...

 http://mama.indstate.edu/pfaffle/yeast/puc12.seq

 http://www.biochemj.org/bj/329/0579/bj3290579.htm#MATMET

 Best of luck,
  Roland



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