rhubner at gins.uia.ac.be
Thu Dec 10 16:41:00 EST 1998
In article <vw3jdBACTAc2Ewn3 at genesys.demon.co.uk>, "Dr. Duncan Clark"
<Duncan at genesys.demon.co.uk> wrote:
> Hi Folks,
> Has anyone any info. on a pUC12 derivative, pUC12N, modified at the lacZ
> ATG to introduce an Nco I site and possibly other modifications. I have
> a plasmid from years ago with an insert that I now need and I can't PCR
> it with universal (-40) and reverse (-48) primers. Looking at pUC12
> those primer sites should still exist but I get no PCR of the 2kb insert
> (which is definitely in there according to RE digests). I have a
> reference for a J. Biotechnol., Vol 2, pp157-175 (1985) but I can't find
> any trace of that journal in three university libraries tried to date.
maybe one of those could help you further? Seems as of ccatgg is the only
Best of luck,
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