purification of 10 to 100 mg of plasmid?

Chris Kafer ckafer at iastate.edu
Fri Dec 11 03:59:44 EST 1998


In article <bpmurray*STUFFER*-1012981449420001 at macmac-2.ucsf.edu>, bpmurray*STUFFER*@socrates.ucsf.edu (Bernard P. Murray, PhD) wrote:
>In article <74pcuj$1gb$1 at nnrp1.dejanews.com>,
>theodorn at medlib.georgetown.edu wrote:
<snip>
>
>> > Why don't you just scale up the Merlin prep.  1 gm of resin can
>> > binds up to 2-3 gm of DNA,
>
>Is this a typo?  Shouldn't that be 2 - 3 milligrams of DNA?

Yes, thats incorrect.  1 g of resin can bind up to 2-3 mg DNA, according to 
the Merlin protocol.


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