in situ hybridization

Dr. Stanislav Kopriva stanislav.kopriva at
Mon Dec 14 10:56:14 EST 1998

Dear netters

Does anyone have a good fixation protocol for in situ of maize leaves ?
We have tried 4% formaldehyde/ 0.25% glutaraldehyde/phosphate buffer for
3h at room temperature or 4% formaldehyde/ 50% EtOH/ 5% acetate 4 h room
temperature. In both cases we are losing the epidermis.
Thanks in advance for all recomendations.

Dr. Stanislav Kopriva
Pflanzenphysiologisches Institut
Altenbergrain 21
CH-3013 Bern
Tel.: +41.31.631 4917
Fax.: +41.31.332 2059

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