RNeasy cleanup of RNA after Dnase treatment

[David Frantz]

   After DNase treatment I try to clean up the RNA with an RNeasy spin
column from Qiagen but I only get about 10% back, that's a 90% loss and
entirely unacceptable!?  Has anyone had a problem with yield?
    I have had success in the past but lately I lose almost all of my
RNA when using the column.  I believe that it does not stick to the
column initially.
    Anybody have a similar experience, or better yet a solution?

-Jefferson

ws3 at byu.edu