RNeasy cleanup of RNA after Dnase treatment

David Frantz djf at EMAIL.BYU.EDU
Tue Dec 15 18:09:56 EST 1998

   After DNase treatment I try to clean up the RNA with an RNeasy spin
column from Qiagen but I only get about 10% back, that's a 90% loss and
entirely unacceptable!?  Has anyone had a problem with yield?
    I have had success in the past but lately I lose almost all of my
RNA when using the column.  I believe that it does not stick to the
column initially.
    Anybody have a similar experience, or better yet a solution?


ws3 at byu.edu

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