Unknown restriction digestion

John Lum lumdicks at netvigator.com
Wed Dec 16 02:40:15 EST 1998


Dear all,

I have a pYES2 vector and I would like to cut the vector with blunt end.
I choose a enzyme called PVUII that would cut the plasmid at 389.
However, I have seen two bands about at 2.8k and 3.1k after digestion
and gel electrophoresis. I don't know what is the problem. Please give
me some idea. Thank you very much!!!



Terry




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