Unknown restriction digestion

.Krimer Alejandro a.krimer at biosidus.com.ar
Wed Dec 16 08:06:35 EST 1998


Jhon Lum wrote:

> I have a pYES2 vector and I would like to cut the vector with blunt
> end.
> I choose a enzyme called PVUII that would cut the plasmid at 389.
> However, I have seen two bands about at 2.8k and 3.1k after digestion
> and gel electrophoresis. I don't know what is the problem. Please give
> me some idea. Thank you very much!!!
> 
In my experience Pvu II has never cut in another sequence. For this
reason I think the only question is that your plasmid has another Pvu II
site. 

Hope this helps,

				Alejandro



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