Sephadex G-50 problem

Long-Cheng li longli at itsa.ucsf.edu
Wed Dec 16 19:56:35 EST 1998


Hi, there

This is the first I used Sephadex G-50 coloum (Promega) to purify r-32P-ATP
labelled oligo probe. After I loaded the sample (10-20ul) and centrifuged
for 5min at 1000g, I got the elute that had hardly any counts as detected by
a Geriger counter. Even I extended the centrifugation time the result was
the same. I don't think my oligo was not labelled at all. Should someone
have the experience of this kind, Please give me a reply. Thanks

Long
Cancer Biology
UCSF



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