TCA precipitation of proteins

[Andrew Pridmore]

Can anyone help with these apparently simple questions, as I cannot find the
answers in textbooks.

When precipitating proteins with TCA, what does the TCA actually do to the
protein structure? In particular I need to know:

1 - The effect on conformation / folding

2 - The effect on bonds within the polypeptides

3 - The effect on electrical charge.

TCA precipitation is a standard technique, but this basic information does
not seem to be readily available. References for further reading would be
much appreciated.

Thanks in advance,

Andrew Pridmore