TCA precipitation of proteins
ampridmore at clara.co.uk
Wed Dec 16 16:04:19 EST 1998
Can anyone help with these apparently simple questions, as I cannot find the
answers in textbooks.
When precipitating proteins with TCA, what does the TCA actually do to the
protein structure? In particular I need to know:
1 - The effect on conformation / folding
2 - The effect on bonds within the polypeptides
3 - The effect on electrical charge.
TCA precipitation is a standard technique, but this basic information does
not seem to be readily available. References for further reading would be
Thanks in advance,
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