Unknown restriction digestion

laurent houzet lhouzet at alize.ulb.ac.be
Wed Dec 16 14:10:59 EST 1998


And What about partieal digestion (2.8 + 0.4 nearly=3.1) ? So is your enzyme
OK?

John Lum wrote:

> Dear all,
>
> I have a pYES2 vector and I would like to cut the vector with blunt end.
> I choose a enzyme called PVUII that would cut the plasmid at 389.
> However, I have seen two bands about at 2.8k and 3.1k after digestion
> and gel electrophoresis. I don't know what is the problem. Please give
> me some idea. Thank you very much!!!
>
> Terry




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