Blunt end ligation problem

Richard P. Grant see_sig at cmtech.co.delete.uk
Thu Dec 17 03:18:35 EST 1998


In article <l03130300b29da97f1480@[128.252.171.220]>,
brett at BORCIM.WUSTL.EDU (Brett Lindenbach) wrote:

> Seems to me like you'd be getting rid of CIP during agarose electrophoresis.
> I do this all the time - no heat, no phenol, just CIP the vector and run the
> reaction on a gel, and I haven't had a problem with CIP'd ends in years.

Super.  

Glad it works for you.  I haven't had a problem with CIP for years because
I use SAP.  ;-)

-- 
Richard P. Grant MA DPhil     |             rgrant at cmtech.co.uk
Senior R&D Scientist          |             work: www.cmtech.co.uk
Cambridge Molecular           |      home: www.avnet.co.uk/adastra           
        -- Hiroshima '45 - Chernobyl '86 - Windows 98 --



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