Blunt end ligation problem
Richard P. Grant
see_sig at cmtech.co.delete.uk
Thu Dec 17 03:18:35 EST 1998
In article <email@example.com>,
brett at BORCIM.WUSTL.EDU (Brett Lindenbach) wrote:
> Seems to me like you'd be getting rid of CIP during agarose electrophoresis.
> I do this all the time - no heat, no phenol, just CIP the vector and run the
> reaction on a gel, and I haven't had a problem with CIP'd ends in years.
Glad it works for you. I haven't had a problem with CIP for years because
I use SAP. ;-)
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
Senior R&D Scientist | work: www.cmtech.co.uk
Cambridge Molecular | home: www.avnet.co.uk/adastra
-- Hiroshima '45 - Chernobyl '86 - Windows 98 --
More information about the Methods