Blunt end ligation problem

Richard P. Grant see_sig at
Fri Dec 18 03:14:19 EST 1998

In article <AyAItAAX8Se2Ewzl at>, "Dr. Duncan Clark"
<Duncan at> wrote:

> There is of course the other way which is not to get rid of CIP but to
> use sodium metavanadate (a potent transition-state analogue inhibitor of
> phosphatases) to inhibit it in all subsequent reactions i.e. during
> ligation or whatever. Sept 1998 Biotechniques pp350-352.

<slaps forehead>The penny drops.  Of course, vanadate is used to
specifically inhibit cellular phospatases, very useful in the cytoskeletal

Richard P. Grant MA DPhil     |             rgrant at
Senior R&D Scientist          |             work:
Cambridge Molecular           |      home:           
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