Blunt end ligation problem

Richard P. Grant see_sig at cmtech.co.delete.uk
Fri Dec 18 03:14:19 EST 1998


In article <AyAItAAX8Se2Ewzl at genesys.demon.co.uk>, "Dr. Duncan Clark"
<Duncan at genesys.demon.co.uk> wrote:

> There is of course the other way which is not to get rid of CIP but to
> use sodium metavanadate (a potent transition-state analogue inhibitor of
> phosphatases) to inhibit it in all subsequent reactions i.e. during
> ligation or whatever. Sept 1998 Biotechniques pp350-352.

<slaps forehead>The penny drops.  Of course, vanadate is used to
specifically inhibit cellular phospatases, very useful in the cytoskeletal
field.

-- 
Richard P. Grant MA DPhil     |             rgrant at cmtech.co.uk
Senior R&D Scientist          |             work: www.cmtech.co.uk
Cambridge Molecular           |      home: www.avnet.co.uk/adastra           
                 -- In Ventum Faciens Aquam --



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