Method for ensuring surgical instruments are RNase-free

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Fri Dec 18 17:07:09 EST 1998


In article <75e62i$acj$1 at nnrp1.dejanews.com>, jdwalls at my-dejanews.com wrote:

>   "Andrew G." <gosbella at vaxc.cc.monash.edu.au> wrote:
> 
> > I am looking for methods for preparing surgical instruments to ensure that
> > they are free of DNA, RNA, DNases & RNases.
> 
> There are a number of commercial products available that will accomplish what
> you want, such as RNAse-Zap, RNAase-off, etc..but since these are durable
> materials, you might consider soaking them in stong basic (pH 8+)solution,
> rinsing in distilled, deionized water, then autoclaving.  The base will
> hydrolyse any nucleic acids, and autoclaving will inactivate any DN/RNAses.
> HTH
> -JW

Nooooo...  RNases laugh at (and often live in) autoclaves.
For steel instruments baking is probably the best way to
go.  There are also some decontamination protocols that
involve soaking in a solution of hydrogen peroxide
(check Sambrook et al.).  Ideally (eg. for excising samples
for RT-PCR) disposable instruments are preferred.
     Have fun,
          Bernard
-- 
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA



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