PCR with supercoiled plasmid

Andrew Leung leungkc at glink.net.hk
Mon Dec 21 12:44:46 EST 1998


Hi all,

    Is there any problem if I amplify a DNA fragment using supercoild
plasmid as template? OR I have to linearize the plasmid piror to PCR? Any
experience on this is greatly appreciated.

Andrew





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