High osmolality of TNM-FH medium

Lee-Feng Chien lfchien at CC3.NDHU.EDU.TW
Wed Dec 23 07:34:12 EST 1998

I am using TNM-FH medium (Grace's medium + yeastolate + lactalbumin
hydrolysate) to culture Sf-21 insect cells. However, the osmolarity of
TNM-FH is about 450 mOsm/kg rather than 350 mOsm/kg reported in the
literature. My insect cells are not happy in this high osmolality medium. I
do not know how to reduce the osmolality of the medium. I will be thankful
for any clue?

Lee-Feng Chien

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