tight regulated vector construction

Guy Tremblay guyt at istar.ca
Wed Dec 23 14:34:05 EST 1998

Dom Spinella wrote:

> The pBAD expression system (sold by Invitrogen -- no relationship, etc.,
> etc.) uses the araBAD promoter and is reportedly pretty tightly
> regulated

Induction/repression ratio:

lac operon, around 50
araBAD operon, around 1500

Thus, araBAD is about 30 times better regulated than lac operon. From my
personal experience, it is very unleaky in the presence of 0.2% glucose.

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