incredible PCR results, who can help explain?

Jongrak Kittiworakarn jongrak at HOTMAIL.COM
Wed Dec 23 21:05:53 EST 1998


One of my friend used to have the same problem.
If you want to increase specificity you may try:
1. Increase the annealing temperature. You may try ordinary PCR ( with 
out touch dowm) first to find the highest possibly annealing temperature 
that you can get your product. Then try increasing your yield by touch 
down PCR in the later cycle.
2. Lower the primer concentration in the first few cycle, then increase 
the concentration for the later cycles.

I think the first one will be more convenient.

Jongrak

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