Ways to amplify GC rich fragment?

Andrew Leung leungkc at glink.net.hk
Fri Dec 25 10:38:44 EST 1998


Hi all,

    I am going to amplify a 1 kb fragment which has 70% GC in content and
with obvious repeated GC stretch. Now, the problem is I can't get it
amplified. Is there any suggestion in this case?

    Specifically, primers I tried has about 60% GC, annealing temperature
61C and with a rather standard PCR condition.

    How should I modify my PCR condition? Thank you.

Andrew





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