Ways to amplify GC rich fragment?

[Ted_Zhang at yahoo.com]

Add betaine, 1M final concentration.
Can be combined with 5% DMSO.


In article <760b1a$h40$1 at unix2.glink.net.hk>,
  "Andrew Leung" <leungkc at glink.net.hk> wrote:
> Hi all,
>
>     I am going to amplify a 1 kb fragment which has 70% GC in content and
> with obvious repeated GC stretch. Now, the problem is I can't get it
> amplified. Is there any suggestion in this case?
>
>     Specifically, primers I tried has about 60% GC, annealing temperature
> 61C and with a rather standard PCR condition.
>
>     How should I modify my PCR condition? Thank you.
>
> Andrew
>
>

-----------== Posted via Deja News, The Discussion Network ==----------
http://www.dejanews.com/       Search, Read, Discuss, or Start Your Own