RNA degredation after DNaseI treatment

Gen Yan Yang gyang at MED.UNC.EDU
Wed Dec 30 11:31:24 EST 1998


Yes, my RNA degreded 25% after I incubate RNA in 65 degress for 15 min
in 5mM MgCl. Do you know the degreded RNA is ended with 5'-phosphate
group or h5'-hydroxylated group?  Thanks in advance!

tfitzwater at NEXSTAR.COM wrote:
> 
> Heat-inactivating the DNase I at 75C will cause metallo-catalyzed cleavage
> of the RNA.  RNA cannot be incubated at elevated temperatures in the
> presence of magnesium.
> 
> Tim Fitzwater
> ---------------------
>

-- 
Gen-Yan  Yang
CB# 7090, 436 Taylor Hall
The University of North Carolina at Chapel Hill
Chapel Hill, NC. 27599-7090 , USA.
Phone:  (919) 966-4436 (work)
        (919) 929-4365 (home)
Email: gyang at med.unc.edu



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