DNA isolation from hair
m3allen at SCIBORG.UWATERLOO.CA
Sun Feb 1 15:43:01 EST 1998
At 04:52 PM 1/30/98 GMT, you wrote:
>I was wondering if someone could send me a protocol for isolating DNA from
>hair follicles. I am searching for a protocol that doesn't require
>anything special other than the average chemicals found in a lab.
>thanks, John Korte
We used this in an undergrad course I was TA' ing, and it seemed to work fine
Place 2-3 hairs with good follicles in 100 ul of extraction buffer.
Incubate at 55 C for 1hour, and then at 95 C for 10 minutes.
Use 7.5 ul per 25 ul PCR reaction (if that's what you're using it for)
50 mM KCl 1.86g
10 mM Tris 0.61g
2.5 mM MgCl2 0.25g
0.1 mg/ml gelatin 0.05g
0.45% NP40 2.25 ml
0.45% Tween 20 2.25 ml
pH to 8.3 at room temperature.
Add ddH2O to 500 ml and autoclave (it will look white until it cools).
Hope this helps
Ph. D. student
University of Waterloo
Waterloo, Ontario, Canada
"Revolution is just a t-shirt away."
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