stripping RNA probes

Otto otto at moon.ovi.ac.za
Mon Feb 2 09:38:39 EST 1998


Hi Judi

Look at:
www.ambion.com/RNA/html/strip-ez.html

 Cheers 
Otto

Judi <9320393 at maties.sun.ac.za> wrote in article
<34D0FAAE.A7FB05ED at maties.sun.ac.za>...
I would like to start hybridizations using RNA probes labelled with DIG
or P-32 but have heard from the previous person who tried it in our lab
that they do not stipp off the membrane very easily. I have to have a
method where I can stripp membranes nicely because I have to probe my
membranes with quite a few probes. I really wouldn't like to run a
million Northerns!!
If anyone has any experience with this please help.
Thanks
Judi





More information about the Methods mailing list