DNA isolation from hair

Michael Allen m3allen at SCIBORG.UWATERLOO.CA
Mon Feb 2 09:23:30 EST 1998


>Date: Sun, 01 Feb 1998 15:35:32
>To: "John Korte" <kortej at css.orst.edu>
>From: Michael Allen <m3allen at sciborg.uwaterloo.ca>
>Subject: Re: DNA isolation from hair
>Cc: methods at net.bio.net
>
>At 04:52 PM 1/30/98 GMT, you wrote:
>>Greetings,
>>I was wondering if someone could send me a protocol for isolating DNA from
>>hair follicles.  I am searching for a protocol that doesn't require
>>anything special other than the average chemicals found in a lab.
>>thanks, John Korte
>>
>
>Hi John
>We used this in an undergrad course I was TA' ing, and it seemed to work fine
>
>Place 2-3 hairs with good follicles in 100 ul of extraction buffer.
>Incubate at 55 C for 1hour, and then at 95 C for 10 minutes.
>Use 7.5 ul per 25 ul PCR reaction (if that's what you're using it for)
>
>Extraction buffer:
>
>50 mM KCl                  1.86g
>10 mM Tris                 0.61g
>2.5 mM MgCl2             0.25g
>0.1 mg/ml gelatin         0.05g
>0.45%  NP40                2.25 ml
>0.45%  Tween 20          2.25 ml
>
>pH to 8.3 at room temperature.
>
>Add ddH2O to 500 ml and autoclave (it will look white until it cools).
>
>Hope this helps
>
>Mike
>



Sorry, I forgot to mention that there is also 6ug of proteinase K in every
reaction

Mike


                     Michael Allen              
                    Ph. D. student

       University of Waterloo
 Waterloo, Ontario, Canada

"Revolution is just a t-shirt away."
			-Billy Bragg




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