FISH problem

F. Vaillant flmv1v at pop-server.cent.gla.ac.uk
Mon Feb 2 05:56:43 EST 1998


Hi,

I have started doing FISH experiment. I have a
problem with the final morphology of the
chromosomes. They appear phffy and enlarged. They
lose any distinctive features sometimes fusing in
one mass. I believe that the denaturation step is
the problem. I have reduced the temperature but
without any significant changes. Is there any
thing that I should do to avoid that? Any
technical help of referal to the appropriate
newsgroup is welcome.

Thanks

F. Vaillant



More information about the Methods mailing list