FISH problem

F. Vaillant flmv1v at
Mon Feb 2 05:56:43 EST 1998


I have started doing FISH experiment. I have a
problem with the final morphology of the
chromosomes. They appear phffy and enlarged. They
lose any distinctive features sometimes fusing in
one mass. I believe that the denaturation step is
the problem. I have reduced the temperature but
without any significant changes. Is there any
thing that I should do to avoid that? Any
technical help of referal to the appropriate
newsgroup is welcome.


F. Vaillant

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