Pichia pains

Michael O'Donohue odonohue at lille.inra.fr
Mon Feb 2 12:08:33 EST 1998


Hi everyone!

Does any have any experience with the larger Pichia vectors (eg pHILS1,
pPIC9 etc.)? I am having a load of trouble trying to clone a fragment
into the EcoR1 /BamH1 sites of pHILS1. Such a simple cloning job!!!! I
have lots of vector, lots of fragment......but not even one miserable
colony. Is transformation efficiency at the root of the problem?

Any ideas?



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