inoue competent cells help !

Robot-Boy s535290 at aix1.uottawa.ca
Tue Feb 3 00:37:13 EST 1998


hey all,

just some quick help. I'm going to try making competent cells the inoue
way, and only had the recipe for making the cells, but not for the actual
transformation. Do I just do the transformation the "regular" way ?
ie.thaw on ice, add DNA, wait 20-30 minutes, heatshock, chill, add SOC
and grow at 37 deg for 60 minutes, plate on selective media.

thanks in advance,

Ed




More information about the Methods mailing list