His-tagged protein, urea & antibody production

[Glenda Lawrence]

Thankyou to everyone who provided information to
my query about urea and antibody production.

Below are a couple more responses that others may
find helpful.

1.
>I have routinely injected histagged proteins resuspended in 8 M Urea into
>rabbits subcutaneously.  The rabbits are fine and the antibody generation
>is good.  I would'nt worry about it.

2.
>There is essentially no problem in injecting urea. It is a normal body
>component, and in fact has been used therapeutically in humans as a
>plasma volume expander (massive quantities infused iv). We have done
>some work in rabbits with protein in 4M urea, and saw no effects, even
>though we caefully looked (even to the point if shaving the inoc site,
>and biopsying for histology).
>
>If urea still worries you, then try using the protein as a suspension
>or precipitate (maybe with an oil-based adjuvant).

Glenda




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Glenda Lawrence PhD
Centre for Virus Research
Westmead Institutes of Health Research
Westmead Hospital
Westmead NSW 2145
Australia

Phone: (61 2) 9845 8086 (Office)
              9885 6363 (Lab)
Fax:   (61 2) 9845 8300
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