inoue competent cells help !

Susan Kang Order at hifax.com
Tue Feb 3 16:05:07 EST 1998


Robot-Boy wrote:
> 
> hey all,
> 
> just some quick help. I'm going to try making competent cells the inoue
> way, and only had the recipe for making the cells, but not for the actual
> transformation. Do I just do the transformation the "regular" way ?
> ie.thaw on ice, add DNA, wait 20-30 minutes, heatshock, chill, add SOC
> and grow at 37 deg for 60 minutes, plate on selective media.
> 
> thanks in advance,
> 
> Ed

Why don't you visit www.hifax.com site? You will get a charming protocol
for making competent cell



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