PCR with degenerate primer

qin ling qinling at medew.nema.wau.nl
Wed Feb 4 11:09:09 EST 1998


I am trying to fish out the cDNA of which 20aa N-terminal seq is known. I
have linkered cDNA as template. So in my PCR, one primer anneals to the
linker, the other one is a set of nested degenerate primers designed based
on aa seq. But I couldn't get any specific bands out of my PCR.
Anybody has done similar experiments with success?  Please give me some
briliant suggestions. 
qin ling

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