removal of hexahistidine tag by peptidase

Georg Lipps Georg.Lipps at uni-bayreuth.de
Wed Feb 4 10:24:12 EST 1998


Hi everybody,

does anyone has been successful in removing a C-terminal of N-terminal
hexahistidine tag by limited exoproteolysis ?
I have tried Aminopeptidase M and Carboxypeptidase A with my protein
but have not been successful. Any hints are greatly appreciated !

Thanks in advance,

Georg



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