Q: differentiating and evaluating different GFPs with fl

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Thu Feb 5 05:48:57 EST 1998


Hi all!

I have done some mutagenesis on the GFP-part of a GFP fusion protein.
Is it possible to screen for positive mutants (higher quantum yield
and / or color shift) by measuring (trypsinized?) cells in a
fluorimeter?

Any ideas, comments, hints and protocols are highly appreciated!

Wolfgang
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Wolfgang Schechinger         
University of Tuebingen
email: wgschech at med.uni-tuebingen.de
http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
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