SeAP as a reporter gene

Jose E. Mejia jmejia at
Fri Feb 6 11:44:08 EST 1998


Has anyone used the human placental secreted alkaline phosphatase (SeAP)
reporter gene system? How did it work in your hands?

I am interested to know if the enzyme activity can be assayed over a very
wide range of expression levels: from residual transcription up to
expression from multiple copies of the SeAP gene.

Many thanks,

J.E. Mejia

Institute of Molecular Medicine
John Radcliffe Hospital
Oxford OX3 9DS, UK
jmejia at

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