stable transfections

ronlab ronlab at uic.edu
Fri Feb 6 12:48:02 EST 1998


Clemens Suter-Crazzolara wrote:

> I would like to make stable transfected
> (HEK or HNK) 293 cells. How much gentamicin
> will I have to use. How much gentamicin
> do you generally use for obtaining
> stable transfected cells ?

And also:

Why do you use gentamicin  to select stable transfectants of 293? What 
marker gene are you using?

Please reply to the newsgroup only.

Eugene Kandel.



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