stable transfections

[ronlab]

Clemens Suter-Crazzolara wrote:

> I would like to make stable transfected
> (HEK or HNK) 293 cells. How much gentamicin
> will I have to use. How much gentamicin
> do you generally use for obtaining
> stable transfected cells ?

And also:

Why do you use gentamicin  to select stable transfectants of 293? What 
marker gene are you using?

Please reply to the newsgroup only.

Eugene Kandel.