PCR with degenerate primer

Dr. J.P. Clewley jclewley at hgmp.mrc.ac.uk
Sat Feb 7 14:07:11 EST 1998

I've had sucess with touchdown and uneven PCR conditions
when using one specific primer and one degenerate one, but
only in nested PCR. If using uneven conditions use the
protocol at and not the one in the
paper below:

1.	Uneven: Chen, X. & Wu, R. Gene 185, 195-199 (1997).
2.	Touchdown: Don, R.H., Cox, P.T., Wainwright, B.J., Baker, K. &
Mattick, J.S. Nucleic Acids Res. 19, 4008 (1991).

I can post the exact conditions I use, if requested.

Jon Clewley

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