protein assay

[Farida Safadi-Chamberlain]

I second this question, since Bradford assay doesn't seem to be sensitive
enough, plus it is not precise from one measurement to another, and time
consuming.  Does anybody have a better method?  Has anybody used the A205
described in Current Protocols in Molecular Biology?

 

On Fri, 6 Feb 1998, Martin Offterdinger wrote:

> Hi !
> Is there anyone who tried the nano orange protein assay from molecular
> probes? I am currently using Bradford, but it is not sensitive enough
> as I have only got 5-8ul of a very dilute sample and would like to get
> an exact protein concentration. Are there any other protein assays
> which are more sensitive than Bradford?
> 
> Martin Offterdinger
> Internal Med.I,Dept. Oncology
> University of Vienna
> Austria
> E-Mail:a8803349.nospam at unet.univie.ac.at
> (remove nospam before mailing)
> 
>