need BL21A(DE3) strain of E. coli

[Daniel Gonzalez]

dalmiabk at phibred.com (bipin k. dalmia) writes:

>hi:

>i need the e.coli strain BL21A(DE3) for expression of a foreign
>protein. this strain is a derivative of BL21(DE3) with the addtional
>mutation dam-13::Tn9.

>anyone know where i can obtain this, preferably commercially?

>thanx, bip

Bipin,

You can obtain that strain as well as HMS174(DE3) from Novagen, their address 
is;

Novagen, Inc. 601 Science Drive, Madison, WI 53711
Phone: 608-238-6110 or 1-800-526-7319
Technical Services: 1-800-207-0144

I seem to remember that they charged very little for a frozen stock.

Good Luck,
Daniel Gonzalez
Rutgers University

>Bipin K. Dalmia, Ph.D.
>Protein Expression and Purification
>Pioneer Hi-Bred International, Inc.
>Johnston, Iowa 50131

>dalmiabk at phibred.com