protein solubilizing out of SDS-PAGE gel

Peter Pediaditakis pxpst2 at vms.cis.pitt.edu
Sun Feb 8 18:17:46 EST 1998


In article <34de0c50.257493920 at 207.126.101.81>, dalmiabk at phibred.com
(bipin k. dalmia) wrote:

> hello:
> 
> i am having trouble detecting a protein on SDS-PAGE gels. this is a
> very very soluble protein and i think that it may be solubilizing out
> of the gel during the staining steps. i am fixing the gel with
> 10%acetic acid/45% methanol and staining with CBB. is there any other
> way to ensure that the protein stays in the gel?

If your protein is abov about 10KD then stick the gel in a blotting
apparatus and transfer it to a PVDF membrane.  Millipore sells some that
have smaller pores and are used for sequencing.

Peter

-- 
"Don't you eat that yellow snow
    WAtch out where the Huskies go"

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