protein solubilizing out of SDS-PAGE gel

bipin k. dalmia dalmiabk at phibred.com
Sun Feb 8 14:53:03 EST 1998


hello:

i am having trouble detecting a protein on SDS-PAGE gels. this is a
very very soluble protein and i think that it may be solubilizing out
of the gel during the staining steps. i am fixing the gel with
10%acetic acid/45% methanol and staining with CBB. is there any other
way to ensure that the protein stays in the gel?

bip


Bipin K. Dalmia, Ph.D.
Protein Core Facility
Pioneer Hi-Bred International, Inc.
Johnston, Iowa 50131

dalmiabk at phibred.com



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