protein solubilizing out of SDS-PAGE gel
peeter.toomik at ut.ee
Mon Feb 9 02:20:26 EST 1998
First, try fixing with 10-12% trichloroacetic acid. If it does not help,
add some 5% of formaldehyde to CBB solution. No preliminary fixing! You
can find the recipe in "Practical Protein Chemistry", A.Dabre (ed.),
Wiley, 1986, which refers to S.Irie et al., Anal. Biochem. 126, 350-354
(1982). Formaline concentration etc. may need some adjustment for
particular protein. This method works O.K. in our lab.
Institute of Molecular and Cell Biology
bipin k. dalmia wrote:
> i am having trouble detecting a protein on SDS-PAGE gels. this is a
> very very soluble protein and i think that it may be solubilizing out
> of the gel during the staining steps. i am fixing the gel with
> 10%acetic acid/45% methanol and staining with CBB. is there any other
> way to ensure that the protein stays in the gel?
> Bipin K. Dalmia, Ph.D.
> Protein Core Facility
> Pioneer Hi-Bred International, Inc.
> Johnston, Iowa 50131
> dalmiabk at phibred.com
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