How to pull-out a specific PCR product?

Annette C. Hollmann ah690549 at bcm.tmc.edu
Mon Feb 9 12:07:13 EST 1998


In article <Pine.SGI.3.94.980208161934.10772A-100000 at leonardo.ls.huji.ac.il> Yoram Gerchman <yoramg2 at leonardo.ls.huji.ac.il> writes:
>Greetings netters.
>Is there a way to "tag" a primer so I could pull-out PCR product that
>contain this primer in one end from a mix of non-specific PCR products
>(biotin maybe?)

exactly.
Put a biotin-tagged 5' base on one primer, do the PCR, and isolate the
product with Dynabeads.
(Dynabeads are streptavidin-coated magnetic beads)

Annette




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